Historical and recent tomato black ring virus and beet ringspot virus isolate genomes reveal interspecies recombination and plant health regulation inconsistencies

Tomato black ring virus (TBRV) and beet ringspot (BRSV) are closely related but distinct members of subgroup B the genus Nepovirus. Both viruses have broad host ranges transmitted by seed, pollen, ectoparasitic nematodes. Although 13 TBRV 3 BRSV genome sequences were already available, no attempt has been made to link sequence data from these recent with those historical isolates studied in pre-sequencing era. High-throughput sequencing was used generate eight new three >60-year-old two >30-year-old isolates, more isolates. These Czech Republic, Germany, UK. We compared all genomes sequenced previously. Intraspecies recombination (three four isolates) frequent amongst genomes. Interspecies also present within RNA1 BRSV-3393 SG GB BRSV-9888 ST GB. No satellite RNAs associated Two commercial enzyme-linked immunosorbent assay (ELISA) kits detect during routine testing differed that one detected only other BRSV, so they likely provide incorrect potentially complementary occurrence information. suggest both ELISA kits, or appropriate molecular tests, be biosecurity authorities avoid this problem. This study illustrates value preserved